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KMID : 0379520000160010063
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한국독성학회지
2000 Volume.16 No. 1 p.63 ~ p.66
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Acute Ethanol Reduces Calcium Signaling Elicited by K+ Depolarization in Cultured Cerebellar Granule Neurons
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Kim Jong-Nam
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Abstract
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The effects of acute ethanol on the high K+ induced Ca^{2+}} signals were examined from primary cultures of cerebellar granule neurons. Ca^{2+}} signals were measured with Calcium Green-1 based microscopic video imaging. Because Ca^{2+}} signal was low in most of granule neurons without stimuli, high KCI was used for depolarization. In most case, acute exposure to ethanol reduced the peak amplitude of the Ca^{2+}} signals, induced by high K+, even though low concentration of ethanol(2~10mM) was used and the effects lasted more than 30min. In was also possible to see differences of ethanol inhibition, i.e. the temporal pattern of Ca^{2+}} signal reductions and the strength of inhibition of Ca^{2+}} signals in cerebellar granule neurons. These results indicate that low concentration of ethanol has diverse actions on the Ca^{2+}} signals in cerebellar granule neurons.
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KEYWORD
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Alcohol, Cerebellar Granule neurons, Intracellular Ca^{2+}}, Calcium Green-1
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